HUMAN SPERMATIDS AND SPERMATOZOA LACKING ACROSOMES
نویسندگان
چکیده
منابع مشابه
The protein encoded by cancer/testis gene D40/AF15q14 is localized in spermatocytes, acrosomes of spermatids and ejaculated spermatozoa.
We have previously identified and cloned a human gene, D40, that is preferentially expressed in testis among normal organs, while it is widely expressed in various human tumor cell lines and primary tumors derived from different organs. In this report, we have examined the expression and localization of this protein in human testis with an antibody specific to D40 protein. In Western analyses, ...
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The r\l=o^\leof acrosomal enzymes of mammalian spermatozoa in sperm penetration through the investments of the ovum and subsequent fertilization has been studied since the development of the method based on treatment ofspermatozoa with the cationic detergent, Hyamine, for detachment of acrosomes (Hartree & Srivastava, 1965). Srivastava, Zaneveld & Williams (1970) modified this method, using Tri...
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The feasibility of fertilization by injecting spermatozoa into oocytes has increased significantly the possibilities for treatment of severe male infertility. However, the rapidity of human application has raised some concern about potential health hazards for the progeny. Human pregnancies and births have also occurred with the use of immature spermatozoa and spermatids, and normal offspring h...
متن کاملSpectrin and ankyrin like proteins in spermatids and spermatozoa of the hamster and some other mammals.
The presence of spectrin and ankyrin-like proteins was investigated during the differentiation and maturation of spermatozoa in mammalian species which have previously been studied for actin and calmodulin. These actin-binding proteins were characterized by immunoblotting and localized by immunoelectron microscopy. Neither spectrin nor ankyrin could be detected in the F-actin rich subacrosomal ...
متن کاملHuman fertilization with round and elongated spermatids.
Human spermatids from ejaculate and testicular tissue have been utilized for evaluating human fertilization by intracytoplasmic sperm injection (ICSI) and, where possible, compared with spermatozoa utilizing sibling oocytes. Round and elongated spermatids obtained from ejaculates were either prepared through Percoll gradients or isolated and washed individually using subzonal insemination needl...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1973
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0350489